This website provides information on the human mammary epithelial cell (HMEC) resources developed in my laboratory and that of Mark LaBarge at the Lawrence Berkeley National Laboratory, University of California. Many of these cell types are made available for distribution. Information is provided on the cell types available, the derivation of these cells, appropriate procedures for cell culture, and some data and models obtained using this HMEC system.
As part of my ongoing research program since 1976, my laboratory has developed and stored frozen a variety of normal, benign, tumor-derived, and in vitro transformed cultures of HMEC. To facilitate scientific investigations using HMEC, our laboratory makes these cells available to other researchers. Descriptions of the cell types available can be found in "Human Mammary Cell Types Generated". A brief introduction to the derivation of these cultures, including our current models of how these HMEC overcome senescence/tumor suppressor barriers to become immortal and malignant can be found in “An Overview”. The “Review” section contains more details of our long-term program; however, this section has not been updated since 2003. I may send personalized PowerPoint file to investigators with more current information. The “Procedures” section contains detailed protocols for how to grow these cells.
Please keep in mind the limits of what we can provide:
If you would like to obtain HMEC from us, you are welcome to contact me for assistance in determining which cells might be the most appropriate for addressing your scientific questions. Contact me (not Tech Transfer) first, by email; further discussions by phone may follow. Please look over the "Human Mammary Cell Types Generated" and “An Overview” sections prior to discussion about cell types to use.
To obtain cells, you will be asked to:
Lawrence Berkeley National Laboratory
1 Cyclotron Road, Bldg. 977
Berkeley CA 94720-8174
Phone-office: 510 486-7273
Phone-AZ home: 928-828-3479
Phone-CA home: 510-339-2032
Phone-Jim Garbe, JCGarbe@lbl.gov: 510 486-6390
Phone-Mark LaBarge, MALabarge@lbl.gov: 510-486-4544
Human Mammary Cell Types Generated
An Overview of Growth, Aging, Senescence, and Immortality
Review of the Human Mammary Epithelial Cell (HMEC) Culture System
(last revised January 2003)
Additional Websites of interest for mammary gland biology:
Biology of the Mammary Gland provided by the National Institute of Health
LaBarge Lab Website
Komen Tissue Bank
This web site was developed with support from the Department of Defense (94MM4530). Additional support has been provided by NIH grants CA24844 and CA112970, and the Director, Office of Science, Office of Biological & Environmental Research, of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.