Fig. 2

Figure 3. Expression of markers associated with proliferation (LI) and senescence (p16, SA-b-Gal) in pre-stasis 184 HMEC with increasing passage. All cells except for 9p-X were grown in M85 with oxytocin; stasis in this population was at passage 15. The 9p-X culture was grown in M85 without oxytocin; stasis was at passage 10. Cultures examined are from the growth curve shown in Fig. 2. Note the reciprocal relationship between the small cells with a positive LI, and the larger, often vacuolated cells (senescent morphology) that are positive for p16 and SA-b-Gal, and negative for LI. Size marker = 200 microns. (4)